Ferroportin expression in Macrophages.

<p>Rat peritoneal macrophages were obtained as described in the methods. The cells were harvested after a 6-hour exposure to 0.1 mg/ml elemental iron from the different iron compounds in the presence or absence of 500nM hepcidin. The data show that exposure to all of the compounds is associated with an increase in ferroportin expression (band at 63 kDa not shown). The iron compound induced ferroportin expression was blunted by the presence of hepcidin. The expression levels were normalized to β-actin.