Total iron content in media and the content of H-ferritin in primary peritoneal macrophages after exposure to the iron formulations.

<p>Rat peritoneal macrophages were obtained by peritoneal lavage. The cells were exposed to 0.1 mg/ml of elemental iron for each of the iron formulations for 24 hours. The media was then monitored for presence of total iron by removing 50uL aliquots from the media over 9 hours. The top figure (A) shows total iron in the media was highest (indicating decreased uptake) for iron sucrose and sodium ferric gluconate (b = not statistically significantly different) at all time points. The other compounds were similar (a) until 7 hours when iron uptake was assessed greater for iron isomaltoside and ferumoxytol (c). There is almost no loss of iron from the media over the time monitored for iron gluconate or iron sucrose. At the end of the experiment, t macrophages were lysed and the amount of H-ferritin present in the cytosol was determined (bottom figure, B). A representative immunoblot for H-ferritin is shown. The bands appear at 21 kDa. The data in the graphs are the mean±SEM of three separate experiments. The asterisk (*) indicates a difference from the control group at p<0.05.