Iron loading modulates LPS-induced <i>hepcidin 1</i> and <i>ferroportin</i> expression in macrophages.

<p>Bone marrow derived-macrophages were incubated with ferric ammonium citrate (FAC, 0, 50 or 100 μM) for 16 hours followed by LPS stimulation (10 ng/mL). <i>Hepcidin 1</i> (<i>Hamp1</i>) and <i>ferroportin</i> (<i>Fpn</i>) mRNA expression were determined after 4 h (<i>Fpn</i>) or 12 h (<i>Hamp1</i>) using quantitative PCR analysis. Data are presented as fold change gene expression relative to the expression in untreated cells. Data are representative of at least three independent experiments and presented as mean ± SD (n = 4). *** p≤ 0.001.