Perturbation of hematopoietic progenitors in the Ts(17¹⁶)65Dn DS mouse model due to trisomy of <i>Erg</i>.

<p><b>A.</b> Relative proportions of GMP, CMP and MEP progenitor subsets within the Lineage^-cKit⁺Sca1^- bone marrow fraction of <i>Erg</i>^{<i>+/Mld2</i>} (n = 12; male = 9, female = 3), <i>Erg</i>^<i>+/+</i> (n = 12; male = 4, female = 8), <i>Ts65Dn/Erg</i>^{<i>+/+/Mld2</i>} (n = 10; male = 4, female = 6) and <i>Ts65Dn/Erg</i>^<i>+/+/+</i> mice (n = 11; male = 7, female = 4). * <i>P</i> < 0.05 compared to <i>Erg</i>^<i>+/+</i>, ** <i>P</i> < 0.05 compared to <i>Ts65Dn/Erg</i>^{<i>+/+/Mld2</i>} by Student's two-tailed t-test. <b>B & C.</b> Analysis of hematopoietic progenitor cells including bipotential erythroid-megakaryocyte progenitor populations as previously defined [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005211#pgen.1005211.ref033" target="_blank">33</a>,<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005211#pgen.1005211.ref034" target="_blank">34</a>]. Representative flow cytometry profiles and gating strategies for analysis of cell populations from bone marrow of <i>Erg</i>^{<i>+/Mld2</i>} (n = 13; male = 4, female = 9), <i>Erg</i>^<i>+/+</i> (n = 10; male = 7, female = 3), <i>Ts65Dn/Erg</i>^{<i>+/+/Mld2</i>} (n = 5; male = 4, female = 1) and <i>Ts65Dn/Erg</i>^<i>+/+/+</i> (n = 7; male = 7) mice are shown. See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005211#pgen.1005211.s001" target="_blank">S1 Table</a> for immunophenotype definitions. (<b>B</b>) The mean number of each population is shown as the percentage of viable bone marrow cells or proportion of Lin^-cKit⁺Sca1^-CD150⁺ cells ± standard deviation. * <i>P</i> < 0.05 compared to <i>Erg</i>^<i>+/+</i>, ** <i>P</i> < 0.05 compared to <i>Ts65Dn/Erg</i>^{<i>+/+/Mld2</i>}. (<b>C</b>) The relative increase or decrease of each progenitor populations compared to wild-type (<i>Erg</i>^<i>+/+</i>) with mean and standard deviations shown, * <i>P</i> < 0.05 compared to <i>Erg</i>^<i>+/+</i>.