Hematopoietic progenitor abnormalities in Ts65Dn(17¹⁶) bone marrow by semi-solid agar clonogenic colony assays.

<p>Numbers of colonies from 25,000 unfractionated bone marrow cells cultured in granulocyte colony-stimulating factor (G-CSF, 10³U/mL), granulocyte-macrophage colony-stimulating factor (GM-CSF, 10³U/mL), interleukin-3 (IL3, 10ng/mL), stem-cell factor (SCF, 100 ng/mL), erythropoietin (EPO, 2U/mL), as indicated, with the type and number of colonies scored after 7 days. The number of biological replicates per genotype is given. Mean and standard deviation shown.</p><p>* = <i>P</i> < 0.05 for comparison with <i>Erg</i>^<i>+/+</i>;</p><p>^§ = <i>P</i> < 0.05 for comparison with <i>Ts65Dn/Erg</i>^{<i>+/+/Mld2</i>} mice by unprotected Fisher’s Least Significant Difference.</p><p>χ2 for difference for a given genotype compared to diploid control <i>Erg</i>^<i>+/+</i> comparing all colony numbers using rounded means shown. Blast, blast colony; G, granulocyte; GM, granulocyte-macrophage; M, macrophage; Eo, eosinophil; Meg, megakaryocyte colony.</p><p>Hematopoietic progenitor abnormalities in Ts65Dn(17¹⁶) bone marrow by semi-solid agar clonogenic colony assays.