The expression and purification of <i>Neq</i>SSB-like from <i>E</i>. <i>coli</i> TOP10F’+pBAD/NeqSSBHT.

<p>The proteins were analyzed on a 12% polyacrylamide gel. Lane M: Unstained Protein Weight Marker (Fermentas, Lithuania), with the molecular mass of proteins marked. Lane 1: soluble protein cell extracts after arabinose induction of protein expression (10 μl). Lane 2: <i>Neq</i>SSB-like after the Ni²⁺-affinity chromatography step (10 μl). Lane 3: <i>Neq</i>SSB-like after His-tag cleavage with TEV protease (10 μl). Lane 4: <i>Neq</i>SSB-like after chromatography on an ssDNA-cellulose column (10 μl).