Results of the analytical gel filtration of <i>Neq</i>SSB-like on the Superdex 75 10/300 GL column.

<p><b>A</b> The standard linear regression curve was generated by plotting the log of the molecular mass of calibration proteins against V_e/V₀ value, namely elution volume divided by void volume. The calibration proteins represented by black triangles include bovine albumin (66 kDa), ovalbumin (43 kDa), carbon anhydrase (29 kDa) and ribonuclease A (13 kDa). <i>Neq</i>SSB-like is represented by the black circle. The regression curve equation and coefficient of determination are shown. <b>B</b> Effects of <i>Neq</i>SSB-like protein concentrations on the elution profiles of gel filtration. The entire range of <i>Neq</i>SSB-like concentrations (12–236 μM) show an elution volume of 15.8 ml corresponding to the monomeric protein.