Impact of A1/A2 depletion and DRB on gene expression.

<p><b>A</b>, The transcriptome of untreated (NS), A1/A2-depleted by RNAi and DRB-treated HCT116 cells was sequenced and reads were assigned. The Venn diagram depicts the number of genes in each sample that display a difference in expression relative to the NS sample, and only includes genes displaying a log2 (fold change) ≥ 2 with a correction for genes expressed at lower level using the following equation: corrected-log2(fold change) = log2(fold change) + 1/gamma^(exp-exp.offset), where gamma = 5 and exp.offset = 0, exp is the average expression level expressed as base 10 log, and fold change is the measured fold change in expression. The <i>p</i>-value of the DRB and ΔA1/A2 overlap is 1.4e-12 (Fisher’s exact test). <b>B</b>, Correlation map plotting the expression changes for the overlapping set of reactive genes in the DRB-treated and siA1/A2-depleted samples. Only genes with fold changes ≥ 2 are considered. The correlation coefficient is 0.692 (Pearson correlation ^2).