Insulin secretory function and glucose tolerance in mouse neonates.

<p>(A-B) Insulin release from neonatal islets (day 4 postnatal) in response to a high-glucose (16.7 mM) challenge. One-way ANOVA followed by Tukey’s <i>post hoc</i> tests were used. (C) Serum insulin concentration in serum collected from neonates (day 4) from each group. One-way ANOVA followed by Tukey’s <i>post hoc</i> tests were performed. (D) IPGTTs were performed on 4-weeks-old pups from each experimental group. Changes in blood glucose level were measured immediately before (t = 0 min), and at four time points after (t = 15, 30, 60 and 120 min), glucose loading. Two-way ANOVA followed by Tukey’s <i>post hoc</i> tests were used. (E) Comparison of areas under the curve (AUCs) across groups. (F) mRNA expression of <i>Ngn3</i> and <i>Insulin</i> in neonatal islets isolated from DX600 or A779 group pups. One-way ANOVA followed by Tukey’s <i>post hoc</i> tests were performed. All data are expressed as means ± SEM, n = 6 per group. *<i>p</i> < 0.05, **<i>p</i> < 0.01 <i>vs</i>. control.