Specificity test for the zfHif-2α and the zfHif-3α antibody.
- Louise Köblitz (751351)
- Birgit Fiechtner (751352)
- Katharina Baus (751353)
- Rebecca Lussnig (751354)
- Bernd Pelster (751355)
Publication date
June 2015
DOIAbstract
<p>A,150 μg total protein of normoxic (lane 2) and hypoxic (lane 3) zebrafish larvae at 5 dpf have been subjected to SDS electrophoresis. Incubation with the zfHif-2α specific antibody results in a band at 93 kD. Preincubation of the antibody with recombinant zfHif-2α protein eliminates this band (lane 5, normoxic, lane 6, hypoxic sample). Lane 1 and 4 show the marker. B, 150 μg total protein of hypoxic (lane 1) zebrafish embryo at 3 dpf have been subjected to SDS electrophoresis. Incubation with the zfHif-3α specific antibody results in a band at 69 kD. Preincubation of the antibody with recombinant zfHif-3α protein eliminates this band (lane 2). Lane 3 shows the marker.</p
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