Characterization of the zFbxo7 protein in zebrafish.

<p>(A) Schematic representation of the zFbxo7 functional domains. The values underneath each domain indicate the amino acids identity between zFbxo7 and hFBXO7 (isoform1). Ubl: ubiquitin-like domain; FP: FBXO7/ PI31 domain; F-box: F-box motif; PRR: proline rich domain. (B) Western blot analysis of the zFbxo7 protein expression at different developmental stages. (C) Western blot analysis of zFbxo7 protein expression in different tissues of eight-month-old adult zebrafish. Actin was used as reference protein.(D) Immunostaining of the zFbxo7 protein in eight-month-old zebrafish brain areas. The zFbxo7 immunoreactivity is shown in brown, while the cell nuclei are counterstained in blue using hematoxylin. The following areas are shown: olfactory bulb (OB), diencephalon (Di), optic tectum (TeO), medulla oblongata (MO), habenula (Ha), and cerebellum (C). Scale bars: 100 µm.