Behaviour of <i>mgtC</i> mutant towards J774 macrophages.

<p>(A) Strain cytotoxicity was evaluated using a LDH assay. A T3SS mutant (Δ<i>pscN</i>) is included as negative control. Error bars correspond to standard deviations from three independent experiments. (B) Survival of bacteria upon phagocytosis. Results are expressed as percentage of surviving bacteria at the indicated time comparatively to the number of bacteria internalized after 30 min of phagocytosis. Error bars correspond to standard errors (SE) from five independent experiments. (C) Effect of inhibition of vacuolar proton ATPase on bacterial survival. The survival of bacteria was measured from macrophages treated or not with bafilomycin A1. Error bars correspond to standard errors (SE) from three independent experiments. In all panels the asterisks indicate <i>P</i> values (Student’s t test, **<i>P</i> <0.01, ***<i>P</i> <0.001).