Silencing of CLDND1 induces death in various breast cancer cell lines.

<p>MDA-MB-231, MDA-MB-468, BT-549, MCF-7 cells were transfected with three different siRNA oligonucleotides targeting CLDND1 or with a control siRNA for 72 h (A-H). Cells were then either subjected to Annexin V-APC staining and flow cytometry analysis (A-D) or analyzed for mRNA expression of CLDND1 with real-time quantitative PCR (E-H). Data in A-D (mean ± S.E.M., n = 3) represent percent AnnexinV-positive cells related to control conditions and in E-F (mean ± S.E.M., n = 2–3) represent CLDND1 mRNA levels normalized to control. Asterisks indicate statistically significant differences (*, p<0.05; **, p<0.01; ***, p<0.001) compared with control cells.