CHD1L promotes chemotaxis of breast cancer cells.

<p>(A) Expression of CHD1L protein in MDA-MB-231 cells (MDA231), MDA-MB-231 cells transfected with control scrambled siRNA (Scr/MDA231), and MDA-MB-231 cells transfected with two sets of stable siRNA-targeting CHD1L (SiCHD1L#1/MDA231 and SiCHD1L#2/MDA231) was detected using Western blot. β-Actin was used as a loading control. Quantification of relative protein levels on three different Western blot analyses is shown below the blots. (B) Comparison of cell proliferation in Scr/MDA231 and SiCHD1L/MDA231 (SiCHD1L#1/MDA231). Each data point was an average of triplicate assays. (C) Cell chemotaxis ability was assessed using chemotaxia assay in MDA-MB-231, Scr/MDA231, and SiCHD1L/MDA231 (SiCHD1L#1/MDA231) with EGF stimulation. Columns, mean of triplicate measurements. Bars, standard deviation. *, <i>P</i> < 0.05 (Student’s t test, versus MDA-MB-231 or Scr/MDA231). (D) Expression of CHD1L protein in MCF-7 cells, MCF-7/Con cells (transfected with GV230 vector), and MCF-7/CHD1L cells (stably transfected with GV230-CHD1L, stable clone 4) was detected using Western blot. β-Actin was used as a loading control. Quantification of relative protein levels on three different Western blot analyses is shown below the blots. (E). Comparison of cell proliferation in MCF-7/Con and MCF-7/CHD1L. Each data point was an average of triplicate assays. (F) Comparison of chemotactic abilities with EGF stimulation in MCF-7, MCF-7/Con, and MCF-7/CHD1L. The data collected in this set of figures are representative of at least three independent experiments. Columns, mean of triplicate measurements. Bars, standard deviation. *, <i>P</i> < 0.05 (Student’s t test, versus MCF-7 or MCF-7/Con).