CLDND1 depletion decreases viability of different breast cancer cell lines.

<p>(A) mRNA expression of CLDND1 in various breast cancer cell lines. MDA-MB-231, MDA-MB-468, BT-549 and MCF-7 cells were incubated under normal conditions for 24 h. Cells were subsequently harvested and were analyzed for mRNA expression of CLDND1 with real-time quantitative PCR. In B-E, MDA-MB-231, MDA-MB-468, BT-549 and MCF-7 cells respectively, were transfected with three different siRNA oligonucleotides targeting CLDND1 or with a control siRNA for 72 h. Cells were thereafter subjected to WST-1 assay. Data (mean ± S.E.M., n = 3) represent the amount of viable cells expressed as percent viable cells obtained under control conditions (B-E). Asterisks indicate statistically significant differences (*, p<0.05; **, p<0.01) compared with control cells.