TLR4 and MyD88 singling pathway is essential for GB-induced DC activation.

<p>C57BL/6, TLR2^-/-, TLR4^-/- or MyD88^-/- mice were injected <i>i</i>.<i>p</i>. with PBS or 50 mg/kg GB for 24 hours. (A) Percentage of lineage^-CD11c⁺ spleen DCs was analyzed on a flow cytometry (left panels). Absolute cell number of spleen DCs within live cells were shown (right panel). (B) Expression level of CD86, MHC class I and MHC class II were measured by flow cytometry. (C) IL-6, IL-12p70 and TNF-α levels in sera were shown. All data are representative or the average of analyses of 6 samples from 3 independent experiments. *<i>p < 0</i>.<i>05</i>, **<i>p < 0</i>.<i>01</i>.