The T cell immune responses in mice immunized with different dosage of LT69.

<p>Mice were immunized subcutaneously with three different doses（50 μg, 10 μg or 2 μg) of LT69 formulated in DDA-Poly I:C three times at 2-wks interval or with a single dose of BCG (5×106 CFU), and the number of vaccine-induced IFN-γ cells was determined at 6 and 24 weeks after the last immunization. Freshly isolated spleen lymphocytes were plated in duplicate at 5×10⁵ cell per well in 96 spot and incubated with ESAT6 (10 μg/ml), Ag85B (5 μg/ml) and HspX (10 μg/ml) for 20h. IFN-γ production was determined using mouse IFN-γ ELISPOT kits. Results are presented as means ± SD, n = 4