Induction of antigen-specific IFN-γ secretion responses in mice immunized with E6E7.

<p>(A) C57BL/6 mice were immunized with three doses of PBS/2M urea (PBS) or E6E7 protein vaccine and challenged with TC-1 tumor cells two w3eeks after the last dose. The ELISPOT assay was performed with PBMC twenty days after the challenge. (B) TLR4 knockout mice were immunized with three doses of PBS/2M urea (PBS) or E6E7 and challenged with TC-1 tumor cells two weeks after the last dose. The ELISPOT assay was performed with PBMC twenty days from the challenge. The cells were stimulated with E6 (VYDFAFRDL^49-57; DKKQRFHNI^127-135) and E7 (RAHYNIVTF^49-57; LCVQSTHVD^67-75) peptides. (C) Comparative IFN-γ production between PBMC stimulated with E6₄₉-₅₇ and/or E7₄₉-₅₇ specific peptides (Stimul.); Ø –Unstimulated cells.