Anti-tumor responses elicited in mice immunized with E6E7 multi-epitope protein.

<p>Mice were immunized with three doses of PBS/2M urea (PBS) or E6E7 in intervals of 14 days. Mice were challenged with TC-1 tumor cells two weeks after the last dose. (A) Groups of five C57BL/6 mice were immunized as described. (B) TLR4 KO mice (C57BL10/ScCr) were vaccinated with PBS/2M urea (5 mice, PBS) or E6E7 (6 mice). (C) Groups of eight CD4 KO mice were vaccinated with PBS/2M urea (PBS) or E6E7. (D) Groups of five CD8 KO mice were vaccinated with PBS/2M urea (PBS) or E6E7. In parallel, C57BL/6 wild-type mice (5 mice) were used as a control of tumor growth competence. Asterisk indicates statistically significant percentage of tumor-free animals when compared to control group (** p < 0.005).