Lower numbers of postmigratory CNCCs after <i>lrp5</i> knock-down results in cranial skeleton malformations.

<p>(<b>A-B’</b>) <i>fli1</i>:EGFP embryos at 30 hpf. (A,A’) Uninjected control embryo, (B,B’) <i>lrp5</i> morphant. Note that mandibular (md), hyoid (hy) and three branchial (br) patches of postmigratory CNCCs are well defined in wild-type but defective in <i>lrp5</i> morphants. (<b>C-F’</b>) <i>fli1</i>:EGFP embryos at 48 hpf. (C,C’) Uninjected control embryo in lateral view, (D,D’) <i>lrp5</i> morphant lateral view, (E,E’) uninjected control embryo ventral view, (F,F’) <i>lrp5</i> morphant ventral view. Note that metameric morphology of pharyngeal arches is absent in <i>lrp5</i> morphant. Only one arch, most likely the 5^th branchial arch is present (ba5?). (<b>G-J’</b>) <i>fli1</i>:EGFP embryos at 72 hpf. (G,G’) Uninjected control embryo in lateral view, (H,H’) <i>lrp5</i> morphant lateral view, (I,I’) uninjected control embryo ventral view (J,J’) <i>lrp5</i> morphant ventral view. Note that in wild-type, cranial elements like Meckel’s cartilage (mc), ceratohyal (ch) and 1^st to 5^th ceratobranchials (cb 1–5) can be distinguished, whereas in <i>lrp5</i> morphant only mc and ch are detectable while cbs are undefined. Anterior is to the left in all images. Boxed areas in X are magnified in X’.