Phenotypes of malformed embryos and fetuses harvested from wild-type pregnant females injected with ZIKV.

<p>(<b>A-B</b>) Embryos from a 12.5 days <i>post coitum</i> (dpc) litter harvested from a C57BL/6J female injected with ZIKV at 5.5 dpc. <b>A</b>, Malformed embryo displaying pericardial edema, unfused mandibular arch, missing hyoid arch, abnormal forelimbs and hindlimb rudiments, consistent with developmental delay and growth restriction (staged at 9.5 dpc, or Theiler stage 15). (<b>B</b>) Outwardly normal littermate of the embryo depicted in <b>A</b> (staged at 11.5 dpc, or Theiler stage 19). (<b>C</b>) Control FVB/NJ embryo from a PBS-injected 12.5 dpc pregnant female (staged at 12.5 dpc, or Theiler stage 20–21). (<b>D</b>) Malformed fetus from an 18.5 dpc litter harvested from a C57BL/6J female injected with ZIKV at 7.5 dpc. Note the abnormal postures of the right fore and left hind limbs, which are reminiscent of arthrogryposis in humans (white arrowheads), with splayed out forelimb and hindlimb digits, typical of 14.5 dpc (Theiler stage 22). In contrast, eye and ear landmarks are compatible with 11.5 dpc (Theiler stage 19), suggesting that the conceptus was already developmentally delayed at the time of its death (see <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0005363#pntd.0005363.s002" target="_blank">S2</a>, <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0005363#pntd.0005363.s005" target="_blank">S5</a> Tables). The malformed conceptus is displayed along with the maternal side of the placenta. (<b>E</b>) Outwardly normal littermate of the fetus in <b>D</b>. The conceptuses were staged at 18.5 dpc (Theiler stages 26–27). (<b>F</b>) Control C57BL/6J fetuses from a pregnant female injected with PBS at 7.5 dpc and harvested at 18.5 dpc. The fetuses were staged at 18.5 dpc (Theiler stages 26–27). Scale bars, 1.0 mm.