Co-localization of cytochrome <i>c</i> and mitochondria in blastocysts cultured for seven days in the absence or presence of DFM.

<p>(A), Blastocysts were labeled with a cytochrome <i>c</i>-specific antibody (green) and MitoTracker (red). (B), Co-localization was analyzed using Image pro plus. (C), Pearson’s R was used to compare the co-localization of cytochrome <i>c</i> and mitochondria. Black bar, control group; white bar, DFM 5.0 μM. Scale bar = 200μm, *<i>P < 0</i>.<i>05</i>. Values are the mean ± SEM of 3–4 independent experiments.