Membrane potential in blastocyst cultured in the absence or presence of DFM.

<p>(A), Membrane potential was calculated as the ratio of red florescence, which corresponds to activated mitochondria (J-aggregates), to green fluorescence, which corresponds to less-activated mitochondria (J-monomers). (B), Fluoresces of each blastocyst were analyses by image pro plus software. The control was arbitrarily set at 1. Black bar, control group; white bar, DFM 0.5 μM; gray bar, DFM 5.0 μM. Values represent means ± SEM from at least three separate experiments. Scale bar = 500 μm. *<i>P < 0</i>.<i>05</i>.