Evaluation of customized TqPCR primers that can be used to assess the RT-reverse transcription quality (RQ) of qPCR templates from mouse RNA samples.

<p><b>(A)</b> Schematic representation of the sizes and locations of the PCR primers (also see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132666#pone.0132666.s002" target="_blank">S2 Table</a>). <b>(B)</b> Comparison and correlation analyses of mean Cq value differences between Bio-Rad’s RQ1 and RQ2 primers (BRQ1 and BRQ2) and customized RQ primers (e.g., MRQ1 through MRQ6). The mean Cq values were obtained by performing TqPCR assay. Possible correlations between BRQ1/2 and MRQ primers were further analyzed statistically.