The Ct values from qPCR for E4 and ML <i>in vitro</i> transcription assays by primer extension under the conditions with or without reverse transcriptase.

<p>No RT LF/LR: <i>In vitro</i> transcription assay with mock primer extension but without reverse transcriptase, then detected by qPCR using luciferase gene primer pair (LF/LR). No RT L/G: <i>In vitro</i> transcription assay with mock primer extension but without reverse transcriptase, then detected by qPCR using luciferase versus GFP gene primer pair (L/G). RT L/G: <i>In vitro</i> transcription assay including primer extension and with reverse transcriptase then detected by qPCR using luciferase versus GFP gene primer pair (L/G). SD: Standard Deviation.</p><p>The Ct values from qPCR for E4 and ML <i>in vitro</i> transcription assays by primer extension under the conditions with or without reverse transcriptase.