RNA-Seq gene quantification and detection closely corresponds to values obtained by species-specific RT-qPCR.

<p>Comparison between RNA-Seq and RT-qPCR across 170 human and 174 mouse genes from a Calu-6 human xenograft sample (Control_2). Correlation in gene expression in (A) human (<i>r²</i> = 0.72) and (B) mouse (<i>r²</i> = 0.60). <i>r²</i> values are calculated only from genes detected on both platforms. Overlap in number of (C) human and (D) mouse genes detected in both RNA-Seq and RT-qPCR, and genes detected on one platform only. The transcript mapped to by the highest number of reads was chosen to represent the expression of its parent gene. Any gene containing at least one mappable read was classed as detected in RNA-Seq. The genes included in this comparison are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066003#pone.0066003.s009" target="_blank">Table S5</a>. Details of gene expression and detection correspondence across both Control_1 and Control_2 samples are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0066003#pone.0066003.s012" target="_blank">Table S8</a>.