DV NS1 protein activates TLR6 of mice.

<p>ELISA was performed to quantify the amount of IL-6 (A) and TNF-α (B) secreted into the supernatant by His-tag (1 μg/ml), DV NS1 recombinant protein (1 μg/ml), MALP-2 (50 ng/ml), Ultrapure LPS (2.5 x 10⁴ EU/ml), mock-infected or DV2-infected (M.O.I of 10) wild-type or TLR6^<i>-/-</i> murine peritoneal macrophages on day 2 post-treatment. (C) Wild-type (23 mice) and TLR6^<i>-/-</i> (9) mice were injected with 20 μg of DV NS1 protein at 1–2 day-old via intraperitoneal injection. Control wild-type (18 mice) and TLR6^<i>-/-</i> (9) mice were injected with 20 μg of His-tag. The survival of the mice was monitored from day 1 to day 7 on a daily basis. Kaplan-Meier survival curves were shown. Log-rank test was performed to determine if there was significant difference in survivability between DV NS1-treated wild-type and TLR6^<i>-/-</i> mice. ELISA was performed to quantify the amount of IL-6 (D) and TNF-α (E) in the livers of wild-type mice injected with 20 μg of DV NS1 protein or His-tag at 1–2 day-old via intraperitoneal injection. Each set of samples was in triplicates.