TLR6 knockout enhanced the survivability of DV2-infected mice.

<p>(A) Wild-type (184 mice) and TLR6^<i>-/-</i> (203) mice were infected with 2.7 x 10⁸ PFU of DV2 strain 16681 at 1–2 day-old via intraperitoneal injection. Mock-infected wild-type (140 mice) and TLR6^<i>-/-</i> (146) mice were injected with 50 μl of C6/36 cell culture supernatant. The survival of the mice was monitored from day 1 to day 21 on a daily basis. Kaplan-Meier survival curves were shown. Log-rank test was performed to determine if there was significant difference in survivability between DV2-infected wild-type and TLR6^<i>-/-</i> mice. The blood and tissues of mice were harvested on day 1, 2, 3, 4, 5 and 21 post-infection via cardiac puncture. The virus in the sera (B), livers (C), limbs (D) and brains (E) of mice were quantified by plaque assays. Virus titers of 0 PFU/ml were set to 0 Log10 PFU/ml to be reflected on the log scale graph. No actual virus titer of 0 Log10 PFU/ml was obtained from any of the samples. Each sample group is in triplicates. Each set of samples was in triplicates.