DV2-infected mice up-regulates IL-6, TNF-α and produces DV NS1 protein.

<p>ELISA was performed to quantify the amount of IL-6 (A) and TNF-α (B) in the sera of mice infected with 2.7 x 10⁸ PFU of DV2 at 1–2 day-old via intraperitoneal injection. There were 24 mice in each time point (12 DV2-infected and 12 mock-infected, 12 wild-type mice and 12 TLR6^-/- mice). Using Kruskal-Wallis test, the amount of IL-6 and TNF-α detected in the sera of DV2-infected wild-type and TLR6^-/- mice were significantly different among the respondents. Wild-type (C) and TLR6^<i>-/-</i> (D) mice at 1–2 day-old were infected with 2.7 x 10⁸ PFU of DV2 strain 16681. DV NS1 protein in the sera of the mice was assayed using Bio-rad Platelia Dengue NS1 Antigen detection kit.