<p>(A) Schematic representation of the position in which sRNAs mapped to the selected genes. Each bar represents the abundance and position of sRNAs mapped to the gene. Small RNAs of 19-22nt (blue bars) and 23-26nt (red bars) are represented. (B) qRT-PCR analysis of mRNAs of selected genes at 2 and 4dpi for TMV-Cg and ORMV-infected plants compared to mock inoculated. Log2 fold change of selected genes at 2 and 4dpi are shown. Errors bars represent SE. * = 0.01≤p≤0.05; ** = 0.001≤p≤0.01. AT5G41740 (LRR1) and AT4G33300 (ADR1) are members of LRRs families; AT1G50440 (E3 RING1) and AT1G11270 (E3 SCF2) are members of E3 ligases families. AT3G43990 (BAH) is a putative transcription regulator. UBQ5 gene was used as a negative control displaying no...
<p>The letter “A” and “B” in the RNA-Seq and qRT-PCR mean the comparison of expression profiles of s...
Supplemental file(s) description: supplementary table 1, supplementary table 2, supplementary table ...
<p>MiRNA expressions from qRT-PCR are shown by line graphs on the top and values are shown on the ri...
<p>(A) Number of genes that produce sRNAs depending on <i>F</i>. <i>graminearum</i> strains. (B) Num...
The differentially expressed transcripts from either the csrA or badR mutant vs. wild-type parent we...
(A) ER stress assay of U. maydis strain JB1 (WT), JB1Δcib1 and UVO151 (Pcrg:clp1). Serial 10-fold di...
<p>(A) Venn diagram comparing sRNA genes that were differentially-expressed in Δ<i>hfq</i> compared ...
<p>The transcriptional start and stop sites of candidate sRNAs were determined by circular RACE. The...
The grey-scale bars represent relative gene expression in control (dark grey) and treated plants (li...
<p>The signal intensity of each unigene was normalised against 18S rRNA as a housekeeping gene. The ...
<p>(A) Total and p19-bound vsiRNAs and endogenous sRNAs derived from <i>N</i>. <i>benthamiana</i> an...
Additional file 6: Figure S2. Negative networks of differentially expressed sRNA candidates and thei...
<p>XY bar charts represent genes selected for real-time PCR validation and their fold changes obtain...
<p>The differential expression of the six sRNAs was analyzed using qRT-PCR in strains M and E3 at 24...
<p>(A) Gene expression data for RNA-seq analysis. (B) The RT-qPCR analysis of gene expression data. ...
<p>The letter “A” and “B” in the RNA-Seq and qRT-PCR mean the comparison of expression profiles of s...
Supplemental file(s) description: supplementary table 1, supplementary table 2, supplementary table ...
<p>MiRNA expressions from qRT-PCR are shown by line graphs on the top and values are shown on the ri...
<p>(A) Number of genes that produce sRNAs depending on <i>F</i>. <i>graminearum</i> strains. (B) Num...
The differentially expressed transcripts from either the csrA or badR mutant vs. wild-type parent we...
(A) ER stress assay of U. maydis strain JB1 (WT), JB1Δcib1 and UVO151 (Pcrg:clp1). Serial 10-fold di...
<p>(A) Venn diagram comparing sRNA genes that were differentially-expressed in Δ<i>hfq</i> compared ...
<p>The transcriptional start and stop sites of candidate sRNAs were determined by circular RACE. The...
The grey-scale bars represent relative gene expression in control (dark grey) and treated plants (li...
<p>The signal intensity of each unigene was normalised against 18S rRNA as a housekeeping gene. The ...
<p>(A) Total and p19-bound vsiRNAs and endogenous sRNAs derived from <i>N</i>. <i>benthamiana</i> an...
Additional file 6: Figure S2. Negative networks of differentially expressed sRNA candidates and thei...
<p>XY bar charts represent genes selected for real-time PCR validation and their fold changes obtain...
<p>The differential expression of the six sRNAs was analyzed using qRT-PCR in strains M and E3 at 24...
<p>(A) Gene expression data for RNA-seq analysis. (B) The RT-qPCR analysis of gene expression data. ...
<p>The letter “A” and “B” in the RNA-Seq and qRT-PCR mean the comparison of expression profiles of s...
Supplemental file(s) description: supplementary table 1, supplementary table 2, supplementary table ...
<p>MiRNA expressions from qRT-PCR are shown by line graphs on the top and values are shown on the ri...