Expression of regional DE miRNAs detected by qRT-PCR and miRNA-seq.

<p>MiRNA expressions from qRT-PCR are shown by line graphs on the top and values are shown on the right Y-axis as relative expression (ΔCt). Lower ΔCt values represent higher miRNA expression levels and <i>vice versa</i>. MiRNA expressions from miRNA-seq are shown by bar graphs on the bottom and values are shown on the left Y-axis as log₂ (normalised reads number). A, B, C - indicate the significant difference in the relative expression of miRNAs detected via qRT-PCR at <i>P</i><0.05; a, b, c - indicate significant difference in the expression of miRNAs detected from miRNA-seq at FDR <0.05. Data are presented as Mean±Standard deviation. RU, MJ and IL represent rumen, mid-jejunum, and ileum, respectively.