Expression of temporal DE miRNAs detected by qRT-PCR and miRNA-seq.

<p>MiRNA expressions from qRT-PCR are shown by line graphs on the top and values are shown on the right Y-axis as relative expression (ΔCt). Lower ΔCt values represent higher miRNA expression levels and <i>vice versa</i>. MiRNA expressions from miRNA-seq are shown by bar graphs on the bottom and values are shown on the left Y-axis as log₂ (normalised reads number). A, B, C, D - indicate significant expression difference between pairs at <i>P</i><0.05 (D7 <i>vs</i> D0, D21 <i>vs</i> D7, D42 <i>vs</i> D21) detected via qRT-PCR. a, b, c, d - indicate significant expression difference between pairs at FDR <0.05 (D7 <i>vs</i> D0, D21 <i>vs</i> D7, D42 <i>vs</i> D21) detected by miRNA-seq. Data are presented as Mean±Standard deviation.