Endogenous and viral sRNAs sequestration preferences of p19.

<p>(A) Total and p19-bound vsiRNAs and endogenous sRNAs derived from <i>N</i>. <i>benthamiana</i> and p19syn plants infected with wt (CymRSV) and suppressor deficient Cym19stop viruses and mock-inoculated plants. (B) input and p19-bound endogenous sRNA duplexes in mock-inoculated plants. (C-F) inputs and p19-bound vsiRNAs and endogenous sRNAs when p19 was expressed in <i>trans</i> (C and D) or <i>in cis</i> (E and F) during virus infections as indicated. The size classes of sRNAs between 18 and 24nt are indicated by numbers The 5’ nucleotides are indicated by color codes. The percentages of specific 5’-nucleotide sRNAs in input and p19 IP are shown in brackets at the right side of the panel (input%; IP%). Note that B, C, E scales differ from D and F ones. Read counts were normalized to 1 million reads.