Co-transcription of <i>ct694</i> and <i>ct695</i>.

<p>(A). The presence of transcripts containing multiple open reading frames was determined by reverse-transcription (RT) PCR with primers surrounding <i>ct694</i> and <i>ct695</i> or <i>ct695</i> and <i>ct696</i>. RNA was isolated from HeLa cells infected with <i>C</i>. <i>trachomatis</i> L2 at an MOI of 0.5 grown to various time points post infection. (B). The same samples were additionally analyzed by quantitative real-time PCR for increased sensitivity. Levels shown are relative to those detected 6 hpi. A Student’s T test with Welch’s correction was employed to assess statistical significance (*, P < 0.04).