HFHSD reduces tissue NAD⁺ levels and CD38 KO mice have elevated tissue NAD⁺ levels.

<p>(A) Tissue NAD⁺ was determined from snap frozen liver, gastrocnemius, brown fat and white fat of WT mice fed a ND (white) or HFHSD (grey) for 5 months. Mice were fasted for 6 hrs before tissue collection. n = 5 per group. ††, p value<0.01 (ND vs HFHSD). See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134927#sec009" target="_blank">Material and Methods</a> for experimental details. (B) Tissue NAD⁺ was measured from snap frozen liver, gastrocnemius, brown fat and white fat of WT (grey) or CD38 KO (black) mice fed with HFHSD before the study. Mice were fasted for 6 hrs before tissue collection. n = 5 per group. *, p value<0.05 (WT vs KO); **, p value<0.01 (WT vs KO).