FBG1-mediated degradation of A1AT-Z also occurs through autophagy.

<p>A1AT-Z was transfected with FBG1 of pCMV in Hepa1-6 cells. 48 hours later the cells were treated with vehicle (H₂O), or autophagy inhibitors for 2.5 hours. A) Representative blot, bottom panel staining for total protein, demonstrates equal amounts of protein were loaded in all the lanes. Upper panel shows increased A1AT-Z after inhibition of autophagy in both pCMV and FBG1 transfected cells. B) ImageJ analysis showing mean values (n = 9) with error bars = SEM of A1AT-Z levels after normalization to total protein.