Altered WWOX modifies ectopic Egr/TNFα-mediated eye phenotype.

<p><b>(A)</b> Ectopic expression of Egr/TNFα (GMR><i>egr</i>^<i>+w</i><i>>empty vector (EV)</i>) results in a decrease in eye size and disruption to ommatidial patterning. <b>(B)</b> The ectopic Egr/TNFα phenotype is completely suppressed by decreased levels of TNFR (GMR><i>egr</i>^<i>+w</i><i>>wengen/TNFR-IR</i>). <b>(C)</b> Decreased expression of WWOX by RNAi knockdown (<i>GMR>egr</i>^<i>+w</i><i>>WWOX-IR</i>^<i>#1</i>) results in suppression of the rough eye phenotype. <b>(D)</b> Quantification of increased eye size with independent WWOX knockdown constructs (<i>GMR>egr</i>^<i>+w</i><i>>WWOX-IR</i>^<i>#1</i> and <i>GMR>egr</i>^<i>+w</i><i>>WWOX-IR</i>^<i>#2</i><i>)</i>. <b>(E)</b> Increased expression of WWOX by ectopic expression of the WWOX cDNA (GMR><i>egr</i>^<i>+w</i><i>>WWOX-cDNA</i>) resulted in an enhancement of the Eiger/TNFα phenotype. <b>(F)</b> Quantification of decreased eye size with independent ectopic expression constructs for WWOX (GMR><i>egr</i>^<i>+w</i><i>>WWOX-ORF</i> and GMR><i>egr</i>^<i>+w</i><i>>WWOX-cDNA)</i>. Significance indicated by **** = p <0.0001, * = p<0.05 and ns = not significant.