Ectopic expression of Egr/TNFα gives increased ROS and is suppressed by increased SOD activity.

<p><b>(A)</b> Ectopic expression of GFP and Egr/TNFα with <i>hh-GAL4</i> in the posterior portion of wing discs of wandering third instar larvae, GFP shows the region of ectopic expression (outlined by dotted line). <b>(B)</b> Punctate CellRox staining revealed increased ROS levels at posterior edge of disc. <b>(C)</b> Merged image with GFP in green and CellRox in red. <b>(D)</b> Control eye phenotype (<i>GMR>EV</i>) showing regular ordered arrays of ommatidial units on the surface of the adult eye <b>(E-F)</b> Ectopic expression of SOD1 or SOD2 alone has no effect. <b>(G)</b>. Ectopic expression of Egr/TNFα in the eye (GMR><i>egr</i>^<i>+w</i><i>>EV</i>) results in a decrease in eye size and disruption to ommatidial patterning. <b>(H-I)</b> The ectopic Egr/TNFα phenotype is obviously suppressed by increased levels of SOD1 or SOD2.