Expression validation of predicted targets in mutant regulator backgrounds.

<p>Root tissue was collected from seedlings grown 4 days on iron sufficient media and transferred to iron deficient media for 3 days. Expression values are normalized to <i><i>β</i>-tubulin</i> and to WT (Col-0) expression for each gene. Error bars indicate ±SEM (n = 4). Mutant backgrounds are (A)<i>obp4-1</i>, (B)<i>wrky57-3</i>, (C)<i>etf9-1</i>, (D)<i>col4-1</i>, (E)<i>asil2-1</i>, and (F)<i>myb55-1</i>. Asterisk indicates significant difference from WT (Student’s t-test, <i>p</i> < 0.05).