Real-Time RT-PCR analysis of transcript levels of iron homeostasis genes.

<p>Multiplexed TaqMan assays were performed for the quantification of iron homeostasis genes as indicated (FAM labeled) and the control, RAD23-3 (VIC labeled) after reverse-transcription of mRNA extracts from control and <i>wbc19</i> mutant plants germinated on MS media with or without 50 mg/l kanamycin. Data shown are from 4 biological replicates and 3 technical replicates. Error bars indicate standard deviation.