Expression of iron-responsive genes in <i>fit-2/</i>35S::FIT:GR plants.

<p>8-day-old plants were grown on 1/2 MS without iron (black) or supplemented with 100 µM Fe (white). Roots were then transferred to liquid media with the same iron content but supplemented as follows: CHX DEX: 100 µM cycloheximide for one hour then 30 µM dexamethasone was added and incubated for 3 hours; CHX: 100 µM cycloheximide for one hour followed by a mock treatment for 3 hours, DEX: mock treated for one hour followed by 30 µM dexamethasone for 3 hours; or mock treatments only. Quantitative RT-PCR results are presented for <i>IRT1</i> (A) <i>FRO2</i> (B) <i>bHLH038</i> (C) and <i>bHLH100</i> (D). Error bars indicate standard error (n = 3–4). Relative transcript levels are shown on the graph.