eGFP-PKC Apl II translocation and recovery from synaptic depression with PDBu from animals >120g.

<p>(A) Representative confocal images of a sensory neuron soma from an animal >120g expressing eGFP-PKC Apl II before and after application of 200nM PDBu. While 10μM 5HT did not result in an increase in the F_mem/F_cyto ratio, 200nM PDBu significantly increased the F_mem/F_cyto ratio at sensory neurons from >120g animals (comparing F_mem/F_cyto before to after with a two-way ANOVA and Bonferroni post tests, 5HT P>0.05, PDBu P<0.05, n = 6 for each group). (B) In a second experiment, also with sensory neurons isolated from animals >120g, 5HT then PDBu were added sequentially with an image before, after 5min in 5HT, and after another 2min in 5HT + PDBu. Again, in this group of sensory neurons, 5HT had little effect on the eGFP-PKC Apl II translocation, however, the subsequent application of PDBu significantly increased translocation (P<0.05 comparing F_mem/F_cyto with 5HT to PDBu with a paired t-test, data from 36 sensory neurons in n = 10 dishes). C) Homosynaptic depression of PSP amplitude and recovery from depression with 5HT or PDBu at synapses formed with sensory neurons from animals >120g. PDBu significantly increases facilitation following depression over 5HT (comparing the data sets with a two-way ANOVA and Bonferroni posttests, PSP1-PSP40 P>0.05 and PSP41-PSP52 P<0.001, n = 9 for 5HT and n = 7 for PDBu). Scale bars are 20<b>μ</b>m.