Longer wash periods lead to greater desensitization of PKC Apl II translocation.

<p><b>A</b>, Representative confocal fluorescence images of sensory neurons expressing eGFP-PKC Apl II during a 3×15 min application of 5HT with 15 min washes in between. <b>B</b>, Quantification of PKC Apl II translocation (bars, n = 9 cells) and modeling output (line). Error bars are SEM. <b>C</b>, Top panel represents S dynamics in response to experimental protocol from <b>A</b>. Black line represents the ratio of S_OFF and S_ON to total S, red line the ratio of S_IN2 to total S, green line represents the ratio of S_AD and S_ADON to total S. Middle panel represents the amount of AD over time and the bottom panel the amount of D over time. Dotted line represents time of second and third 5HT application. <b>D</b>, Representative confocal fluorescence images of sensory neurons expressing eGFP-PKC Apl II during a 3×15 min applications of 5HT with 25 min washes in between. <b>E</b>, Quantification of PKC translocation (bars, n = 6 cells) and modeling output (line). Error bars are SEM. <b>F</b>, Top panel represents S dynamics in response to experimental protocol from <b>E</b>. Black line represents the ratio of S_OFF and S_ON to total S, red line the ratio of S_IN2 to total S, green line represents the ratio of S_AD and S_ADON to total S. Dotted line represents time of second and third 5HT application. Middle panel represents the amount of AD over time and the bottom panel the amount of D over time.