Dental Infection of <i>P</i>.<i>g</i>. Induces Defects in Placental Tissues in Mice.

<p>Representative histological findings in gd 15-placental tissue from the NC group (A-D) and <i>P</i>.<i>g</i>.-infected group (E-H) by H&E staining (n = 6 for each group). (A, E) Overview of mouse placenta: La, Labyrinth; Sp, Spongiotrophoblasts; De, Decidua; Ut, Uterus wall. (B, F) Epithelium of amnion: in the <i>P</i>.<i>g</i>.-infected group, amnion epithelium (black arrow) is degenerative and detached from chorionic plate (white arrow). (C, G) Labyrins and Spongiotrophoblasts layers: Trophoblasts and endothelial cells are necrotic (*) in <i>P</i>.<i>g</i>.-infected group. (D, H) Decidua and uterus wall: Placental abruption is evident in the <i>P</i>.<i>g</i>.-infected group. ** shows the separation between placenta and uterus at maternal-fetus junction. (I) Immunolocalization of <i>P</i>.<i>g</i>. (brown pigments) (n = 6 for each group); 1000x-magnification by oil-immersed microscopy. (J) Gene expression for the <i>mgl</i>-gene of <i>P</i>.<i>g-</i>W83 strain by nested PCR; representative results from 6 mice for each group. PC, positive control. Scale bars, 100μm. Olympus BH2 microscope and Nikon digital sight DS-L2 camera were used for capturing images.