TIMP-2 activation of both ERK 1/2 and AKT is mediated by Ras.

<p><b>A</b>. MT1-MMP-expressing Tet-Off MCF-7 cells transiently transfected with RasN17 cDNA or control, empty vector were incubated with TIMP-2 (100 ng/ml) for 15 min. Cell extracts were analyzed by Western blotting for ERK1/2 and AKT activation (p-ERK1/2; p-AKT), and for RasN17 expression using β-tubulin (TUB) as a loading control. B. Densitometric analysis. *, <i>p</i> ≤ 0.05, + TIMP-2 <i>vs</i>. the corresponding – TIMP-2 sample; # RasN17 <i>vs</i>. the corresponding empty vector control. This experiment was repeated three times with comparable results.