ERK1/2 but not AKT activation by TIMP-2/MT1-MMP is dependent on FGF receptor.

<p>Western blotting analysis of AKT and ERK1/2 activation (p-AKT; p-ERK1/2). MT1-MMP-expressing Tet-Off MCF-7 cells pretreated with the indicated concentrations of PD173074 or control vehicle (0) for 15 min were incubated with (<b>A</b>) recombinant FGF-2 (10 ng/ml; Akron Biotech, Boca Raton, FL, USA) or (C) TIMP-2 (100 ng/ml), or with control medium (-), for additional 15 min. <b>B</b> and <b>D</b>. Densitometric analysis of the blots shown in A and B, respectively. Total AKT and ERK2 are shown as loading controls. *, <i>p</i> ≤ 0.05, <i>vs</i>. the corresponding control with no PD173074. This experiment was repeated twice with comparable results.