TIMP-2 induces AKT activation in a dose- and time-dependent manner.

<p>Western blotting analysis of AKT activation (pAKT) in MT1-MMP Tet-Off MCF-7 cells incubated with (<b>A</b>) TIMP-2 (100 ng/ml) for the indicated times or (<b>B</b>) with the indicated concentrations of TIMP-2 for 15 min. The cells shown in panel A were grown in the absence of DOX; the cells shown in panel B were grown either in the presence (-MT) or in the absence (+MT) of DOX. Total AKT is shown as a loading control. <b>C</b> and <b>D</b>. Densitometric analysis. *, <i>p</i> ≤ 0.05, <i>vs</i>. time 0; #, <i>p</i> ≤ 0.05, + MT1-MMP <i>vs</i>. the corresponding – MT1-MMP sample. These experiments were repeated twice with similar results.