<p>Cells were incubated with PYC (10, 25, 50 μg/mL) or DMSO (vehicle) for 1 h before addition of LPS (500ng/mL) for 24h (A) or cells were incubated with PYC (50 μg/mL) or vehicle in indicated time before or after LPS stimulation for 24h (B). The culture supernatants were collected and analyzed for nitrite production using Griess reagent and a standard curve was created using NaNO<sub>2</sub> in culture medium. Data are represented as mean ± SD from at least 3 independent experiments. *P< 0.05 compared with LPS alone. <sup>#</sup>P< 0.05.</p
<p>The NO production was indirectly determined by measuring nitrite level in the cultured supernatan...
<p><b>A–B.</b> Nitric oxide (NO) assay analysis indicates that nitrite level is significantly increa...
<p>Cells were incubated with the indicated concentrations of PYC or vehicle for 1h before 24 h LPS t...
<p>Cells were incubated with the indicated concentrations of PYC or vehicle for 1h before LPS treatm...
<p>BV2 cells were treated with PYC at various concentrations or DMSO (vehicle) for 1h. Then, LPS (50...
<p>Peritoneal macrophages from BALB/cmice were incubated in the absence or presence of 1 µg/mL LPS a...
<p>BV2 cells were treated with PYC at various concentrations or DMSO (vehicle) (10, 25, 50 μg/mL) fo...
<p>(A) NF-κB-luciferase reporter plasmids were transfected in BV2 cells for 4 h, pre-treated with PY...
<p>After stimulation for 24 h with 10 ng/ml LPS a dose dependent decrease of NO production in cells ...
<p>Cultured cells were treated with LPS (100 ng/ml) or PBS (control; Cont.) for the indicated time p...
<p>RAW 264.7 cells were challenged with LPS and LrS obtained from bacteria grow in PETG medium in ea...
<p>The cells were pretreated with poly(P)<sub>130</sub> (100 µM) for 5 min and stimulated with LPS (...
<p>Cells (5 X 10<sup>5</sup> cells/well in 24-well pate) were treated with LPS (100 ng/mL) only and ...
<p>The BV2 cells were transfected with either MSS1-specfic or control siRNA for 24 h, then cells wer...
<p>BV2 cells were treated with PYC or vehicle at the indicated concentrations for 1h. LPS (500ng/mL)...
<p>The NO production was indirectly determined by measuring nitrite level in the cultured supernatan...
<p><b>A–B.</b> Nitric oxide (NO) assay analysis indicates that nitrite level is significantly increa...
<p>Cells were incubated with the indicated concentrations of PYC or vehicle for 1h before 24 h LPS t...
<p>Cells were incubated with the indicated concentrations of PYC or vehicle for 1h before LPS treatm...
<p>BV2 cells were treated with PYC at various concentrations or DMSO (vehicle) for 1h. Then, LPS (50...
<p>Peritoneal macrophages from BALB/cmice were incubated in the absence or presence of 1 µg/mL LPS a...
<p>BV2 cells were treated with PYC at various concentrations or DMSO (vehicle) (10, 25, 50 μg/mL) fo...
<p>(A) NF-κB-luciferase reporter plasmids were transfected in BV2 cells for 4 h, pre-treated with PY...
<p>After stimulation for 24 h with 10 ng/ml LPS a dose dependent decrease of NO production in cells ...
<p>Cultured cells were treated with LPS (100 ng/ml) or PBS (control; Cont.) for the indicated time p...
<p>RAW 264.7 cells were challenged with LPS and LrS obtained from bacteria grow in PETG medium in ea...
<p>The cells were pretreated with poly(P)<sub>130</sub> (100 µM) for 5 min and stimulated with LPS (...
<p>Cells (5 X 10<sup>5</sup> cells/well in 24-well pate) were treated with LPS (100 ng/mL) only and ...
<p>The BV2 cells were transfected with either MSS1-specfic or control siRNA for 24 h, then cells wer...
<p>BV2 cells were treated with PYC or vehicle at the indicated concentrations for 1h. LPS (500ng/mL)...
<p>The NO production was indirectly determined by measuring nitrite level in the cultured supernatan...
<p><b>A–B.</b> Nitric oxide (NO) assay analysis indicates that nitrite level is significantly increa...
<p>Cells were incubated with the indicated concentrations of PYC or vehicle for 1h before 24 h LPS t...