PYC suppressed LPS-induced NF-κB activity and DNA binding of AP-1 in BV2 microglia.

<p>(A) NF-κB-luciferase reporter plasmids were transfected in BV2 cells for 4 h, pre-treated with PYC 10, 25, and 50 μg/mL or DMSO (vehicle) for 1 h, then challenged with 500ng/mL LPS for 6 h. NF-κB activity was expressed as relative luciferase activity. *P< 0.05 compared with LPS alone. Cells were treated with indicated doses of PYC or DMSO (vehicle) for 1 h before 500ng/mL LPS treatment for 30 min, and then subjected to Western blot to detect p65 protein levels (B) and EMSA to assess AP-1 activation (C). ³²P-end-labeled AP1 consensus oligonucleotide was used as a probe. Unlabeled oligonucleotide was used as a competitor. The experiment was repeated at least 3 times and similar results were obtained.