Decreased iNOS expression and NO production by MSS1 gene silencing in LPS-activated microglia.

<p>The BV2 cells were transfected with either MSS1-specfic or control siRNA for 24 h, then cells were stimulated for 24 h with LPS (1000 ng/mL). At 24 h post-LPS incubation, cell lysates were analyzed for the protein production of iNOS using western blot. The Griess assay was performed to measure the production of the NO metabolite, nitrite. Transfection with MSS1-specific siRNA suppressed the LPS-induced iNOS expression at protein levels, along with the production of nitrites Data were obtained from three independent experiments with four to six replicates each. *<i>p</i><0.05 compared with the negative control group.